1) Oct 4th, 2017: 90 min, Graduate School of Humanities room 408
Introduction to the “Essential Bioinformatics for Global Leader I” classes and general bioinformatics concept [style: lecture, discussion]
-Program of the classes, expectation of students
-History, general definition/overview of bioinformatics and its applications in different fields of science.

2) Oct 11th, 2017: 90 min, Graduate School of Humanities room 408
Basics of molecular biology I [style: lecture, discussion]
Introduction to the cell, organization and composition, cells in their context (models of cells, cancer, infection, development, tissue renewal…)

3) Oct 18th, 2017: 90 min, Graduate School of Humanities room 408
Basics of molecular biology II [style: lecture, discussion, computer practice]
PROTEIN, structure/function, analytical techniques and tools, proteomics databases exploration

4) Oct 25th, 2017: 90 min, Graduate School of Humanities room 408
Basics of molecular biology III [style: lecture, discussion, computer practice]
DNA, structure, basic genetic mechanisms (RNA, mutation, SNP, gene repair, replication, protein translation, evolution…..), analytical techniques and tools, genomics databases exploration.

5) Nov 1st, 2017: 90 min, Graduate School of Humanities room 408
Basics of molecular biology IV [style: lecture, discussion]
Omics and Bioinformatics; new molecular techniques, application and social implication of biological investigation, discussion over scientific papers.

6) Nov 8th, 2017: 90 min, Graduate School of Humanities room 408
RT-qPCR, primer construction [style: lecture, discussion, computer practice]
NCBI sequence database, how to make primers and PCR (NCBI Database/sequence alignment)

7-8) Nov 15th, 2017: 180 min, Science building 1, Practice room 521
RNA isolation [style: wet lab]
RNA extraction from animal tissue/cells (Guanidinium thiocyanate-phenol-chloroform method), RNA quantitation assessment (Nanodrop)

9) Nov 22nd, 2017: 90 min, Science building 2 room 202
RNA quality assessment [style: wet lab]
Bioanalyzer use+ sample preparation for next experiments

10) Nov 29th, 2017: 90 min, Science building 2 room 202
Gene expression analysis [style: wet lab, computer practice, discussion]
RT-qPCR (data analysis using thermal cycler software, PCR products gel electrophoresis)

11-12) Dec 6th, 2017: 180 min, Science building 1, Practice room 521
Protein expression analysis I [style: wet lab]
Protein extraction from animal tissue/cells, protein quantitation, protein separation by acrylamide gel electrophoresis (western-blotting)

13-14) Dec 13th, 2017: 180 min, Science building 1, Practice room 521
Protein expression analysis II [style: wet lab, computer practice, discussion]
Semi-quantitative western-blotting (immunoblotting, Image J data analysis)
Conclusion/discussion

15) Dec 20th, 2017: 90 min, Graduate School of Humanities room 408
Example of small scale high-throughput transcriptomic methods (PCR array), discussion over scientific paper.